In vitro maturation of immature human oocytes in culture medium containing follicular fluid

Ali, Jaffar and A.J, Halim and Arshat, Hamid (1987) In vitro maturation of immature human oocytes in culture medium containing follicular fluid. Malaysian Journal of Reproductive Health, 5 (1). pp. 23-25. ISSN 0217-3213

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Abstract

In clinical IVF programs often immature oocytes are retrieved. Under such circumstances, numerous workers have advocated the procedure of pre-incubation in insemination medium for 12-36 hours, prior to insemination to induce maturation. We describe here the supplementation of follicular fluid in insemination medium to induce maturation of immature oocytes. The culture characteristics of the insemination medium containing follicular fluid appears to be better than the insemination medium without follicular fluid. Previous reports suggest the presence of a meiosis-inducing substance in follicular fluid which could trigger the resumption of meiosis and hence contribute to oocyte maturation. Other factors in follicular fluids which are yet to be elucidated, may also contribute to oocyte maturation. It is concluded that superior culture characteristics can be obtained if immature oocytes are preincubated for 16-36 hours in insemination medium containing 50% follicular fluid.

Item Type: Article
Keywords: IVF, culture medium, embryo transfer
Subjects: 600 Technology > 610 Medicine & health > 618 Gynecology, obstetrics, pediatrics & geriatrics
Division/Agency/State: LPPKN - National Population and Family Development Board, Malaysia > Division of Reproductive Health
Deposited By: Mrs Noor Azreena Abd Aziz
Deposited On: 29 Apr 2015 02:32
Last Modified: 06 Feb 2017 04:17
URI: http://familyrepository.lppkn.gov.my/id/eprint/510

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