||600 Technology > 610 Medicine & health
||LPPKN - National Population and Family Development Board, Malaysia: Human Reproductive Division
||Hormone changes, Oviductal fluid
||The hard copy can be refer at Resource Center, NPFDB.
||To detect hormone induced changes in oviductal fluid protein pattern, flushings obtained from ovariectomized untreated and estradiol or estradiol plus progesterone treated monkeys were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Superimposed densitometric scans of coomassie blue and periodic acid Schiff's (PAS) stained electrophoretic patterns revealed that estradiol treatment results in the appearance of a new protein (130 K) with maximum carbohydrate content as compared to other proteins present in the luminal milieu. The concentration of 85 and 95 K proteins also increase significantly after estradiol treatment. However, progesterone supplementation in estradiol primed monkeys results in the disappearance of 85 and 95 K proteins and decreased the 130 K glycoprotein considerably. Thus while estrogen acts as an inducer,progesterone antagonizes its action in monkey fallopian tubes and inhibits the synthesis of proteins which may have a role in early reproductive events.