Browse by Subject
Results for Search : "610 Medicine & health"
|
National experience in population matters: adolescents and youth
Item Type: Newsletter
Editor:
Year: 00/00/2012
Abstract: Adequate and accurate sexual and reproductive health information helps young people to make informed and responsible decisions. Howewer, cultural and religious sensitivities within the communities remain one of the major challenges in implementing reproductive health education for young people. Parents are still uncomfortable talking about reproductive health matters with their children ,leaving their children dependent upon information from their peers or other sources like the internet. The Government of Malaysia takes cognisance of the increasing importance of the sexual and reproductive health needs of adolescents and youth in Malaysia. Hence, the Government of Malaysia has put in place various policies and programmes to handle the sexual and reproductive health needs of the adolescents and youths. One of the main initiatives is the Healthy Programme without AIDS for Youth (PROSTAR). The Government of Malaysia also has established six youth-friendly adolescent centres known as kafe@TEEN to increase access to reproductive health information and services for young people aged 13 to 24 years.
|
|
|
|
|
|
The 44th session of the Commission on Population and Development on agenda item 4: general debate on national experience in population matters: fertility, reproductive health and development, 11th April, 2011
Item Type: Country Statement
Editor:
Year: 11/04/2011
Abstract: Fertility among Malaysian women is declining faster than expected due to the increase in the average age at first marriage for women that has risen from 24.7 years in 1991 to 25.3 in 2004. Malaysia's total fertility rate (TFR) is expected to reach the replacement level by year 2015. With more and more women participating in the labour force and prioritising career development, this has also led to many highly educated women choosing to marry late or not to marry at all.
Malaysia hopes to sustain the current fertility rate and not let it fall below replacement level. Sustaining the current TFR of 2.3 is one of the major challenges that has to be monitored as it can lead to contraction of labour supply, rapid aging of the population and other social implications.
|
|
|
|
|
|
Cytotoxicity and expression profiles of apoptosis gene related in human cervical cancer (HeLa) cell lines in response to Newcastle disease virus (NDV) strains AF2240 And V4-UPM
Item Type: Thesis
Editor:
Year: 01/01/2011
Abstract: In this study the cytotoxicity and expression profiles of apoptosis gene related in human cervical cancer (HeLa) cell lines in response to Newcastle disease virus (NDV) strains AF2240 and V4-UPM were studied. NDV is a strain of avian paramyxovirus. NDV has been classified into the order Mononegavirales, family Paramyxoviridae, subfamily Paramyxovirinae and genus Rubulavirus. NDV caused severe economic losses in the poultry industry worldwide. Several local strains of Newcastle disease virus were reported to induce cytolysis to the cancerous cell lines. Strain AF2240 is a heat resistant viscerotropic velogenic NDV and strain V4-UPM is a heat resistant lentogenic which has significant higher thermostabilities of infectivity and haemagglutination were reported cytolysis leukemic cells in vitro and has shown in vivo anti leukemic agents . In this study the cytotoxicity effects of strains of NDV AF2240 and V4-UPM towards HeLa cell were determined by using standard microtetrazolium assay (MTT). Cytotoxicity dose 50% (CD50) cells treated with different titre of NDV as haemagglutination units (HAU) as compared to the untreated cells was estimated at 72 hours post-infection. The CD50 values obtained were 0.95 HAU and 1.0 HAU for strains AF2240 and V4-UPM, respectively. No cytolytic effect was noted towards normal cells (3T3) was observed. Both strains were also observed to inhibit HeLa cell proliferation. Morphological observations also have been done under inverted light and fluorescence microscopes. Under the inverted light microscope, the HeLa cells treated with both strains showed apoptotic features such as cell shrinkage, cell blebbing and formation of apoptotic bodies. Morphological features of apoptosis were also observed by using the AO/PI staining method under the fluorescence microscope. The AO/PI staining demonstrated the occurrence of apoptosis which was characterised mainly by chromatin condensation, nuclear shrinkage and formation of apoptotic bodies. Evidently, both strains AF2240 and V4-UPM used in the study were found to induce cells towards apoptosis rather than necrosis. NDV strain AF2240 and strain V4-UPM was also caused genotoxic in HeLa cells after two hours treatment with CD10 and CD25 values by alkaline comet assay. Results showed that HeLa cells treated with NDV strains AF2240, V4-UPM and hydrogen peroxide gave different distribution of scores. The HeLa cells treated with hydrogen peroxide as a positive control gave more percentage at score 2, 3 and 4 for both cytotoxicity values compared to the HeLa cells treated with NDV for both strains. Observation in this study has proved the genotoxic potential of the NDV strains AF2240 and V4-UPM to induce DNA damage on HeLa cells as early as two hours following treatment at very low cytotoxicity dose (CD10 and CD25) values. Meanwhile, the cell cycle analyses of HeLa cells treated with local strains of NDV AF2240 or V4-UPM did not induce cell cycle arrest in any specific phase. Sub-G1 phase (apoptosis peak) was found in both treated cells with a very high percentage compared to untreated cells with a small percentage. The results indicate that, the percentages of apoptosis were significantly increased (p≤0.05) in the time-dependent manner in both NDV strains treated HeLa cells. The molecular mechanisms of apoptosis may depend on the NDV strain and cell type. Six apoptosis genes were selected in this study namely Casp8, TNF-α, Bcl2 and TRAIL which focused on extrinsic pathway of apoptosis, while the gene Bax was used as an indicator for intrinsic pathway triggered by cellular stress. Lastly Myc, oncogene was used as an indicator for cell growth. From this study, NDV strain AF2240 was identified as a highly induced death receptor pathway due to the upregulation of TNF gene and the downregulation of Bax gene. Whereas NDV strain V4-UPM triggered both pathways but through the extrinsic pathway due to the very high expression of the TNF gene. The TNF gene was highly expressed due to its location and function as a stimulator of the death receptor pathway. The Casp8 gene was activated and expressed in order to enter the execution-phase of cell death. The Bcl-2 gene was continuously observed because of its function as an apoptosis regulator. Surprisingly, no expression was detected by the TRAIL gene. NDV strain AF2240 was more effective than NDV strain V4-UPM as an apoptosis inducer. These gene expression results showed that the apoptosis occurred and lead to cell death.
|
|
|
|
|
|
Kajian faktor-faktor penghalang wanita berkahwin menjalani ujian pap smear
Item Type: Research Report
Editor:
Year: 00/00/2010
Abstract: Cervical cancer is the second most dangerous threat to women that not only lead to a loss of pregnancy, but can even be life threatening. 12.9 percent of all cancer deaths among women are also due to this cancer. Unfortunately, 80% of women who are first diagnosed with cervical cancer are already at serious stage and cannot be treated properly even though cervical cancer screening programs such as Pap Smear have existed since 1943. Statistics released by the Ministry of Health Malaysia through the 2003 National Cancer Registry Report showed, even after the implementation of the Pap Smear program, the incidence rate of cervical cancer has increased where in 1990 the incidence rate was 13.3 per 100,000 women compared to 19.7 per 100,000 women in 2003. This illustrates that the Pap Smear program in Malaysia is not comprehensive and not practiced by those who need it which is women who have started sexual activity.
Therefore, the National Population and Family Development Board (LPPKN) has conducted a study in relation to cervical cancer screening or Pap Smear test. This study aimed to provide information on sociodemographic characteristics and reproductive history, women’s awareness on pap screening tests, women’s risk perceptions of cervical cancer, women’s attitudes towards the importance of Pap Smear testing and prevalence of Pap Smear screening test practice. This cross -sectional study was conducted in the Klang Valley, Melaka, Perak and Terengganu using purposive sampling. The total sample was 1000 people. The study population consisted of once married women aged between 20-65 years. This includes married women, widows and widowers. The conditions for rejecting the sample are ineligible women, such as unmarried women, non-Malaysian citizens and women who have had cervical cancer.
The collection of research data is through interviews based on the text of the questionnaire provided. Respondents will be interviewed by interviewers who have been trained to obtain accurate information. To achieve the objectives of the study, the analysis used is descriptive analysis, chi-square test and t test. Therefore, based on the analysis that has been conducted, the prevalence of female Pap Smear test is 55.2 percent. Respondents showed a positive attitude when asked about the importance of Pap Smear test compared to other examinations, many of whom thought that this test was very important (92.7 percent). Only 4.2 percent considered the Pap Smear test not important and there were a few who could not provide certainty about its importance (3.1 percent). Not many can measure the risk they are facing to get cervical cancer as 377 (41.5 percent) people are unaware of the cancer risk they are facing. However, there were a total of 276 (30.4 percent) women who were confident that they were not at risk at all. Only 7.7 percent received a high risk of this cancer, moderate risk (5.6 percent) and low risk (14.9 percent).
|
|
|
|
|
|
Tingkah laku seks di kalangan warga 45 tahun dan ke atas
Item Type: Research Report
Editor:
Year: 00/00/2006
Abstract: An opinion survey on 'Sexual Behavior Among Citizens 45 Years and Above' which was first conducted by the Human Reproduction Division (BRM) was conducted in the Klang Valley area from February to June 2003. The 'Convenience Sampling' sampling method was used where respondents who meet certain criteria fill in the questionnaire themselves. A total of 473 respondents consisting of 50.1% Malays, Indians (26.6%) and Chinese (23.3%) where men (53.5%) outnumber than women (46.5%). The majority of respondents comprised the age group of 50-59 years (41.25%). From the 220 female respondents, only 7.3% took hormone replacement therapy. The results of the study found that the importance and satisfaction of sex decreases with age where it is more pronounced among women than men. The frequency of sexual intercourse is performed in the range of 4-8 times a month (46.3%). Although only 16.7% of respondents had sex-related problems, 70.9% of them did not know the source of help and treatment for their problems. Among those who received treatment, 56.4% chose to seek modern treatment. 55.7% of respondents with sexual problems have at least one chronic disease. A total of 85.6% disagreed and looked for another partner as a way out of sex -related problems. Expectations for better sex in the future are still high at 69.1%. In conclusion, for middle-aged and above despite the declining importance of sexual intercourse, their right to access to knowledge and services related to sexual problems needs to be addressed immediately, especially by medical practitioners, especially the Human Reproduction Division and LPPKN in general. A systematic and ‘gender-sensitive’ approach can help overcome the problem of family institutional breakdown and problems related to incest.
|
|
|
|
|
|
Delayed marriage and the fertility crisis in East and Southeast Asia
Item Type: Conference or Workshop Item
Editor:
Year: 00/00/2006
Abstract: The fertility transition in Asian Countries is part of the worldwide fertility transition and one of the most fundamental events of the last half of the 20th century. In general, this has been associated with greatly increased child survival and changes in the economy and society resulting from economic development. But there have been puzzling differences between countries. The fertility crisis used to refer to the countries with high fertility but now attention has shifted to those with very low fertility, a number of which are in Asia.
|
|
|
|
|
|
The National Study on reproductive health and sexuality of adolescents in Malaysia 1994/1995
Item Type: Research Report
Editor:
Year: 00/00/1996
Abstract: The National Study on Reproductive Health and Sexuality of Adolescents in Malaysia was initiated with the aim of formulating a National Programme on Reproductive Health for Adolescents. The specific objectives of the study are:
i. to establish the status of the knowledge, attitude
and practice of adolescents with regard to sexual
and reproductive health,
ii. to identify the constraints that adolescents face in
seeking information and service relating to sexual
and reproductive health,
iii. to obtain the participation of adolescents in the
design and implementation of programmes and
activities for their own welfare.
The single most expected outcome of the Study on Reproductive Health and Sexuality of Adolescents in Malaysia is a Cabinet memorandum which will propose appropriate policy and programme changes for the promotion and maintenance of optimal reproductive health among Malaysian adolescents geared towards the year 2020. This Memorandum will serve as a vital national reference and benchmark for evaluating future trends and patterns. It will be the basis for the reorientation of values, attitudes and approaches for the management of sexuality and reproductive health of adolescents in the country.
|
|
|
|
|
|
The detection of estrogen-dependent proteins in monkey oviductal fluid
Item Type: Article
Editor:
Year: 00/12/1990
Abstract: To detect hormone induced changes in oviductal fluid protein pattern, flushings obtained from ovariectomized untreated and estradiol or estradiol plus progesterone treated monkeys were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Superimposed densitometric scans of coomassie blue and periodic acid Schiff's (PAS) stained electrophoretic patterns revealed that estradiol treatment results in the appearance of a new protein (130 K) with maximum carbohydrate content as compared to other proteins present in the luminal milieu. The concentration of 85 and 95 K proteins also increase significantly after estradiol treatment. However, progesterone supplementation in estradiol primed monkeys results in the disappearance of 85 and 95 K proteins and decreased the 130 K glycoprotein considerably. Thus while estrogen acts as an inducer,progesterone antagonizes its action in monkey fallopian tubes and inhibits the synthesis of proteins which may have a role in early reproductive events.
|
|
|
|
|
|
A role for cholesterol synthesized de novo by human umbilical cord
Item Type: Article
Editor:
Year: 00/12/1990
Abstract: We have previously shown that the human umbilical cord in-vitro possess the ability to synthesize isocaproic acid and presumbly pregnenolone from cholesterol. Here, we examined the ability of the cord to synthesize cholesterol from acetate. Homogenates of umbilical cord (36-41 weeks gestation)obtained following spontaneous vaginal delivery from uncomplicated pregnancies (age 22-31 years) were incubated with [2-14C] acetate. Using the reverse-isotope dilution procedure, cholesterol was isolated and characterized as [14C] cholesterol acetate. Confirmation of identity of newly synthesized cholesterol was obtained after recrystallisation with added non-radioactive cholesterol acetate as standard. The rate of 14C incorporation is small but definite. The results indicate that the homogenates of human term umbilical cord contain the enzymes responsible for the conversion of [2-14C] acetate to [14C] cholesterol. It is suggested that cholesterol synthesized de novo can serve as a percursor for progesterone synthesis in-vivo.
|
|
|
|
|
