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PUBLICATIONS



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The detection of estrogen-dependent proteins in monkey oviductal fluid
Item Type: Article
Author: 
A., Paliwal and
V.P., Kamboj and
B., Malaviya and
Author: 
Editor: 
Year:  00/12/1990
Abstract:  To detect hormone induced changes in oviductal fluid protein pattern, flushings obtained from ovariectomized untreated and estradiol or estradiol plus progesterone treated monkeys were analysed by sodium dodecyl sulphate polyacrylamide gel electrophoresis. Superimposed densitometric scans of coomassie blue and periodic acid Schiff's (PAS) stained electrophoretic patterns revealed that estradiol treatment results in the appearance of a new protein (130 K) with maximum carbohydrate content as compared to other proteins present in the luminal milieu. The concentration of 85 and 95 K proteins also increase significantly after estradiol treatment. However, progesterone supplementation in estradiol primed monkeys results in the disappearance of 85 and 95 K proteins and decreased the 130 K glycoprotein considerably. Thus while estrogen acts as an inducer,progesterone antagonizes its action in monkey fallopian tubes and inhibits the synthesis of proteins which may have a role in early reproductive events.
 
 
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A role for cholesterol synthesized de novo by human umbilical cord
Item Type: Article
Author: 
A, Loganath and
R., Gunasegaram and
K.L., Peh and
P.C.T., Chew and
S.S., Ratnam and
Author: 
Editor: 
Year:  00/12/1990
Abstract:  We have previously shown that the human umbilical cord in-vitro possess the ability to synthesize isocaproic acid and presumbly pregnenolone from cholesterol. Here, we examined the ability of the cord to synthesize cholesterol from acetate. Homogenates of umbilical cord (36-41 weeks gestation)obtained following spontaneous vaginal delivery from uncomplicated pregnancies (age 22-31 years) were incubated with [2-14C] acetate. Using the reverse-isotope dilution procedure, cholesterol was isolated and characterized as [14C] cholesterol acetate. Confirmation of identity of newly synthesized cholesterol was obtained after recrystallisation with added non-radioactive cholesterol acetate as standard. The rate of 14C incorporation is small but definite. The results indicate that the homogenates of human term umbilical cord contain the enzymes responsible for the conversion of [2-14C] acetate to [14C] cholesterol. It is suggested that cholesterol synthesized de novo can serve as a percursor for progesterone synthesis in-vivo.
 
 
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Coagulation profile in women on low-dose oral contraceptive pills
Item Type: Article
Author: 
Ishak, Roshidah and
Hassan, Khalid and
Yusof, Baharum and
Author: 
Editor: 
Year:  00/12/1990
Abstract:  A cross-sectional study looking at the coagulation system was carried out involving 175 women attending the National Population and Family Development Board's Clinic at the Maternity Clinic, General Hospital, Kuala Lumpur. Study subjects comprise of 50 combined low-dose estrogen/progesterone oral contraceptive (OC) pill users and 75 non-OC users, acting as controls. The subjects were on the pill for a period of one year or more. There were significant shortening of the prothrombin time (PT) and partial thromboplastin time (PTT) in the OC group as compared to the control group. However, the activities of factors II, V and VII assayed were not significantly different between the two groups, suggesting that the changes in the PT and PTT were not significant clinically. The effect of long term usage of combined low-dose OC pills does not seem to indicate changes in the coagulation profile of the women in our study.
 
 
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